Analisis filogenetik genus mus berdasarkan gen cytochrome b (cytb) menggunakan metode maximum likelihood
Abstract
Phylogenetic analysis is essential to understand evolutionary divergence and relatedness among species within the genus Mus. This study aimed to examine the phylogenetic relationships of 15 Mus species based on cytochrome b (Cytb) using the Maximum Likelihood (ML) method, with Rattus norvegicus as an outgroup. DNA sequences were retrieved from GenBank and analyzed in MEGA 12. The resulting tree revealed major clades separating Eurasian and African lineages. Mus musculus, M. spicilegus, M. macedonicus, and M. cypriacus formed a well-supported clade with short evolutionary distances, indicating close relationships. Asian species such as M. caroli and M. saxicola formed a distinct branch with greater genetic divergence. African species, including M. minutoides, M. musculoides, M. mattheyi, M. indutus, and M. haussa, clustered into a separate lineage that diverged substantially from the Eurasian group. The varying branch lengths captured by ML reflect heterogeneous substitution rates among sites.
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References
Boursot, P., Auffray, J. C., Britton-Davidian, J., & Bonhomme, F. (1993). The evolution of house mice. Annual Review of Ecology and Systematics, 24(1), 119–152. https://doi.org/10.1146/ANNUREV.ES.24.110193.001003/CITE/REFWORKS
Dharmayanti, I. (2011). Filogenetika Molekuler : Metode Taksonomi Organisme. 30, 1–10.
Kumar, S., Stecher, G., Li, M., Knyaz, C., & Tamura, K. (2018). MEGA X: Molecular evolutionary genetics analysis across computing platforms. Molecular Biology and Evolution, 35(6), 1547–1549. https://doi.org/10.1093/molbev/msy096
NCBI. (2025). National Center for Biotechnology Information. https://www.ncbi.nlm.nih.gov/#!/about
Phifer-rixey, M., Harr, B., & Hey, J. (2020). Further resolution of the house mouse ( Mus musculus ) phylogeny by integration over isolation-with-migration histories. 9, 1–9.
Roy, S. S., Dasgupta, R., & Bagchi, A. (2014). A Review on Phylogenetic Analysis : A Journey through Modern Era. September, 39–45.
Zhou, X., Shen, X., Hittinger, C. T., & Rokas, A. (2017). Evaluating Fast Maximum Likelihood-Based Phylogenetic Programs Using Empirical Phylogenomic Data Sets. 35(2), 486–503. https://doi.org/10.1093/molbev/msx302
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