Isolasi dan identifikasi senyawa golongan flavonoid dari kelopak bunga rosella (Hibiscus sabdariffa, Linn) menggunakan metode sonikasi
Abstract
Rosella flower is a plant that contains anthocyanins. Anthocyanins are included in the flavonoid compound which has a conjugated double bond system that functions as an antioxidant. The purpose of this study was to determine how to isolate flavonoid compounds, the results of phytochemical tests, and the characterization of rosella (Hibiscuss sabdariffa, Linn) flower flavonoids using a UV-Vis spectrophotometer, FTIR, GC-MS, and 1HNMR. The method used is the sonication method. The extraction results using the sonication method obtained a red concentrated extract with a yield of 37.57% from 25 grams of rosella. The result of the hydrolysis process is the formation of CO2 bubbles with side products in the form of H2O and NaCl salt. The result of the partition process is the formation of 2 layers, namely the organic phase and the water phase with a yield of 14.61%. In this study, the results of the solution were orange (brownish orange) indicating a positive result for flavonoids through a phytochemical test. KLTP monitoring results are in the form of yellow spots with Rf 0.7125. The results of characterization using UV-Vis obtained a wavelength of 268.9 nm. The results of the FTIR obtained some typical absorption of functional groups from flavonoids. The results of the GC-MS characterization obtained a peak with a retention time of 20.377 which is the peak of the flavonoid compound.
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References
Afifah, N. (2013). Aktivitas Antibakteri Kombinasi Gentamisin dan Ekstrak 10 Tanaman Obat Terhadap Bakteri Pseudomonas Aeruginosa dan Methicillin Resistant Staphylococcus Aureus (MRSA). Journal of Chemical Information and Modeling, 53(9).
Alfaridz, F., & Amalia, R. (2018). Klasifikasi dan Aktivitas Farmakologi dari Senyawa Aktif Flavonoid. Farmaka, 16(3), 1–9.
Artini, N. P. R., Mahardiananta, I. M. A., & Nugraha, I. M. A. (2022). Rancang Bangun Chiller Berbasis Mikrokontroler Untuk Evaporasi Senyawa Bahan Alam. Jurnal Resistor, 5(1), 10–16.
Djaeni, M., Ariani, N., Hidayat, R., & Utari, F. D. (2017). Ekstraksi Antosianin dari Kelopak Bunga Rosella (Hibiscus sabdariffa L.) Berbantu Ultrasonik: Tinjauan Aktivitas Antioksidan. Jurnal Aplikasi Teknologi Pangan, 6(3), 148–151.
Fasya, A. G. (2016). Ekstraksi, Hidrolisis dan Partisi Metabolit Sekunder dari Mikroalga Chlorella sp . Journal of Chemistry, 5(1), 5–9.
Fitrya, Anwar, L., & Fitria Sari. (2009). Identidikasi Flavonoid dari Buah Tumbuhan Mempelas. Jurnal Penelitian Sains, 12(3C), 1–5.
Gandjar, I. G. dan Rohman, A. 2007. Kimia Farmasi Analisis. Yogyakarta: Pustaka Pelajar.
Handoko, D. S. (2006). Kinetika Hidrolisis Maltosa pada Variasi Suhu dan Jenis Asam Sebagai Katalis. SIGMAL Jurnal Sains dan Teknologi, 9 (1).
Ikalinus, R., Widyastuti, S., & Eka Setiasih, N. (2015). Skrining Fitokimia Ekstrak Etanol Kulit Batang Kelor (Moringa Oleifera). Indonesia Medicus Veterinus, 4(1), 77.
Inggrid, M., Hartanto, Y., & Widjaja, J. F. (2018). Dwicahyani, U., Isrul, M., & Noviyanti, W. O. N. (2019). Formulasi Sediaan Lipstik Ekstrak Kulit Buah Ruruhi (Syzygium policephalum Merr) Sebagai Pewarna. Jurnal Mandala Pharmacon Indonesia, 5(02), 91–103. Jurnal Rekayasa Hijau, 2(3), 283–289. https://doi.org/10.35311/jmpi.v5i02.48
Lasang, M. B., Aisyah, A., Iswadi, I., & Bakar, A. N. A. (2019). Pengaruh Kandungan Senyawa pada Ekstrak Daun Jati (Tectona grandis) Terhadap Nilai Efisiensi Dye Sensitized Solar Cell (DSSC). Teknosains: Media Informasi Sains Dan Teknologi, 13(1), 45–54. https://doi.org/10.24252/teknosains.v13i1.9615
Prayudo, A., Novian, O., Setyadi, & Antaresti. (2015). Koefisien Transfer Massa Kurkumin dari Temulawak Ayndri. Jurnal Ilmiah Widya Teknik, 14(1), 26–31.
Rahim, A. (2016). Hidrolisis Selulosa dari Bahan Pod Husk Kakao. 4(6), pp. 702–711.
Rajalakshmi, D & S. Narasimhan. (1985). Food Antioxidants: Sources and Methods of Evaluation dalam D.L. Madhavi: Food Antioxidant Technological, Toxilogical and Health Perspectives. Marcel Dekker Inc., Hongkong: 76-77.
Redha, A. (2010). Flavonoid: Struktur, Sifat Antioksidatif, dan Peranannya dalam Sistem Biologis. Jurnal Berlin, 9(2), 196–202. https://doi.org/10.1186/2110-5820-1-7
Rohman A. 2009. Kromatografi untuk Analisis Obat. Yogyakarta: Graha Ilmu.
Rusdiana, I. A., Hambali, E., & Rahayuningsih, D. M. (2018). Pengaruh Sonikasi Terhadap Sifat Fisik Formula Herbisida yang Ditambahkan Surfaktan Dietanolamida. Agroradix, 1(2), 34–41.
Sari, W. E. T. (2008). 050800352.pdf. Malang: Universitas Brawijaya.
Setiawan, I.M.A. (2008). Isolasi dan Identifikasi Senyawa Golongan Flavonoid pada Ekstrak n-butanol Kulit Batang Bungur (Lagerstroemia speciosa Pers.). Skripsi. Denpasar: Universitas Udayana.
Suradji, S. I., Najib, A., & Ahmad, A. R. (2016). Studi Komparasi Kadar Flavonoid Total pada Bunga Rosella Merah (Hibiscus sabdariffa L.) Asal Kabupaten Luwu Utara Provinsi Sulawesi Selatan dan Kabupaten Kediri Provinsi Jawa Timur. Jurnal Fitofarmaka Indonesia, 3(2), 175–181. https://doi.org/10.33096/jffi.v3i2.219
Wiradnyani, N. K., & Wartini, N. M. (2014). Komposisi Senyawa Penyusun Minuman Sinom (curcuma domestica val.- tamarindus indica l.). Media Ilmiah Teknologi Pangan, 1(1), 10–23.
Yuniati, W. W., Khairul, A. Dewi, K. (2012). Isolasi, Karakterisasi dan Uji Aktivitas Antioksidan Flavonoid dari Ekstrak Air Kulit Batang Ketapang Kencana (Terminalia muelleri Benth.). Jurnal Sains Dan Matematika, 20(3), 71–76.
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